Journal: PLoS ONE

Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

doi: 10.1371/journal.pone.0007475

Figure Lengend Snippet: PBMC were cultured in SFM at 5×10 5 cells/ml in 8-well glass slides for 7 days. Cells were then air-dried, fixed, and stained with antibodies. A) CD13, B) CD14, C) CD45RA, D) CD45RB, E) CD45RO, F) Irrelevant mouse IgG2a control. Cells were then counterstained with hematoxylin to identify nuclei. Positive staining was identified by red staining, with nuclei counterstained blue. Solid arrow points to a fibrocyte, open arrow points to a macrophage, and asterisk indicates a cluster of lymphocytes. Photomicrographs are representative results from at least four different donors. Bar is 50 µm.

Article Snippet: Cellular Fibronectin , Fn-3 , Mouse IgG1 , Cymbus Biotechnology.

Techniques: Cell Culture, Staining, Control

Journal: PLoS ONE

Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

doi: 10.1371/journal.pone.0007475

Figure Lengend Snippet: PBMC were cultured as described in . Cells were then air-dried, fixed, and stained with antibodies against A) CD18, B) CD68, C) CD163, D) CD206, E) CD209, and F) mouse IgG1 control. Cells were then counterstained with hematoxylin to identify nuclei. Bar is 50 µm.

Article Snippet: Cellular Fibronectin , Fn-3 , Mouse IgG1 , Cymbus Biotechnology.

Techniques: Cell Culture, Staining, Control

Journal: PLoS ONE

Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

doi: 10.1371/journal.pone.0007475

Figure Lengend Snippet: PBMC were cultured in SFM at 5×10 5 cells/ml in 8-well glass slides for 7 days in the presence of 10% FCS and 10 ng/ml M-CSF. Cells were then air-dried, fixed and stained with 5 µg/ml mouse IgG1 monoclonal antibodies against CD13, CD14, CD32, CD163, CD90, mouse IgG1, proyly-4-hydroxylase, 25F9, S100A8/A9, PM-2K, TE-7, and collagen-I. Cells were then counterstained with hematoxylin to identify nuclei. Inserts indicate staining at higher magnification. Bar is 50 µm.

Article Snippet: Cellular Fibronectin , Fn-3 , Mouse IgG1 , Cymbus Biotechnology.

Techniques: Cell Culture, Staining

Journal: PLoS ONE

Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

doi: 10.1371/journal.pone.0007475

Figure Lengend Snippet: PBMC were cultured in SFM at 5×10 5 cells/ml in 8-well glass slides for 1 hour ( ex vivo ) or 7 days. Cells were then air-dried, fixed, and stained with antibodies against A and B) CD64, C and D) CD32, E and F) CD16, G and H) CD32a, I and J) CD32b, or K and L) goat IgG control antibodies. Cells were then counterstained with hematoxylin to identify nuclei. Asterisks are to the right of macrophages. Bar is 50 µm.

Article Snippet: Cellular Fibronectin , Fn-3 , Mouse IgG1 , Cymbus Biotechnology.

Techniques: Cell Culture, Ex Vivo, Staining, Control

Journal: PLoS ONE

Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

doi: 10.1371/journal.pone.0007475

Figure Lengend Snippet: PBMC were cultured as described in . Normal human dermal fibroblasts were cultured for 2 days in 8-well glass slides. Cells were then air-dried, fixed, and stained with antibodies. A and B) collagen-I, C and D) collagen IV, E and F) proyly-4-hydroxylase, G and H) rabbit IgG control antibody. Cells were then counterstained with hematoxylin to identify nuclei. Asterisks are to the right of macrophages. Bar is 50 µm.

Article Snippet: Cellular Fibronectin , Fn-3 , Mouse IgG1 , Cymbus Biotechnology.

Techniques: Cell Culture, Staining, Control